Major Equipment
1. Peptide Synthesis
Applied Biosystems 433A:- Single peptide, batch synthesizer with Fmoc and t-Boc chemistry capability at a scale of 0.1 to 0.5 mmol.
Applied Biosystems 430A:- Single peptide, batch synthesizer with Fmoc and t-Boc chemistry capability at a scale of 0.1 to 0.5 mmol. Fast cycles allow a 20mer peptide to be synthesized in 8h.
Perseptive Biosystems Pioneer:- Fmoc chemistry only, continuous flow instrument, which will synthesize 2 peptides simultaneously.
Rainin Symphony:- Multiple peptide synthesizer using batch synthesis methodology and either Fmoc or t-Boc chemistry. Up to 12 peptides can be synthesized simultaneously.
Advanced Chemtech APEX396:- Multiple peptide synthesizer using batch synthesis methodology and either Fmoc or t-Boc chemistry. Number of peptides that can be synthesized at one time depend on the RV block used and the desired synthesis scale.
Peptide Institute Hydrogen Fluoride Apparatus:- Permits the safe handling of HF required for final cleavage and deprotection of t-Boc synthesized peptides.
2. Mass Spectrometry
Applied Biosystems QSTAR XL QTOF:- High sensitivity, high resolution hybrid MS with nanospray and o-MALDI sources. This instrument is optimized for MS/MS experiments making it useful for PTM analysis by MS.
Micromass (Waters Corp) LR Reflectron MALDI-TOF:- High sensitivity, high resolution MALDI-TOF with reflectron capability.
VG (Waters Corp) Quattro II:- This triple quadrupole MS is ideal for routine intact mass analysis of peptides and proteins up to approx. 70kD, when sample amount is not critical.
LC Packings capillary/nano- flow HPLC:- with Probot fraction collector. This can be used as a standalone LC or used to infuse samples for LC-MS.
3. Protein Sequencing
Applied Biosystems 494:- We have two 4-channel Edman protein sequencers, one of which is dedicated for handling P32-labelled samples.
