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Human P450c17
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1. Structure-function studies of human P450c17 and P450c21
The objectives of this project include determining the key residues of these enzymes responsible for their unique activities; the biochemical and biophysical mechanisms involved; and a detailed understanding of how cytochrome b5 selectively stimulates the 17,20-lyase activity of P450c17. |
2. Cellular and biochemical mechanisms that regulate steroid flux
This project endeavors to elucidate mechanisms beyond gene expression that determine, at a cellular level, the relative flux of precursors along pathways to different classes of active steroid hormones. |
3. Protein engineering of hydroxysteroid dehydrogenases (HSDs)
Although HSDs catalyze the reversible interconversion of hydroxy- and keto-steroids in vitro, each enzyme appears to function unidirectionally in vivo. The goal of this project is to determine general rules that determine HSD directionality and the roles of specific physical properties of these enzymes in their functions. |
4. Molecular genetics of steroid biochemistry
The pathways and enzymes responsible for the disposition of large amounts of certain steroid hormones remains enigmatic, and we will attempt to clarify these processes in animal models and in human beings. |
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| | Auchus Lab | |
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| Richard Auchus |
| | | Lab Interests | |
| | | Lab Members | | Web page under construction
| | | Positions Available | | Please email Richard Auchus for more information.
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