The flow cytometry multi-user core facility has both north and south campus locations. The facility is equipped with two FACScans, two FACSCaliburs, a CyAn, LSRII, and a Luminex-100 for user operated data acquisition.  Users are trained to run the benchtop analyzers and billing is calculated in half hour increments.

• FACScan - single laser instrument, 488nm,  capable of three fluorescent colors
• FACSCalibur - dual laser instrument with lasers at 488nm and 635nm, capable of four fluorescent colors *
• CyAn - a three laser, nine color instrument, with lasers at 488nm, 405nm, and 635nm
• LSR II - a four laser, sixteen color instrument, with lasers at 488nm, 532nm, 405nm, and 635nm laser *
  * The Calibur and LSRII on the north campus are both equipped with a HTS, high throughput system, to acquire sample directly from a 96 or 384 well plate.
  

There are three sorters available for use in the facility, the FACStar PLus, MoFlo, and Aria.  Facility staff performs all sorting.

• MoFlo - three laser, nine color, high speed sorter with lasers at 488nm, 633nm, and 360nm
• Aria - a five laser, eighteen color, high speed sorter with lasers at 488nm, 532nm, 405nm, 635nm

Training is mandatory for access to the benchtop analyzers.  Training is free and should be scheduled when an experiment is available.
Computers and multiple software packages are available for data analysis free of charge to users.

Location 1:
Jake L. And Nancy B. Hamon Biomedical Research Building
North Campus NA7.300
6000 Harry Hines Blvd.
Dallas, TX 75390-9093
214-648-7325

Location 2:
Cecil H. and Ida Green Biomedical Research building,
South Campus Y7.310
5323 Harry Hines Blvd.
Dallas, TX 75390-9093
214-648-6887