The flow cytometry multi-user core facility has both north and south campus locations. The facility is equipped with two FACScans, three FACSCaliburs, a CyAn, and a LSR II for user operated data acquisition.  Users are trained to run the benchtop analyzers and billing is calculated in half hour increments.

•   FACScan - single laser instrument, 488nm,  capable of three fluorescent colors
•   FACSCalibur - dual laser instrument with lasers at 488nm and 635nm, capable of four fluorescent colors *
•   CyAn - a three laser, nine color instrument, with lasers at 488nm, 405nm, and 635nm

        LSR II - a four laser, sixteen color instrument, with lasers at 488nm, 532nm, 405nm, and 635nm laser *
* The Calibur and LSRII on the north campus are both equipped with a HTS, high throughput system, to acquire sample directly from a 96 or 384 well plate.

There are five cell sorters available for use in the facility, the MoFlo, and three Arias are operated by facility staff only.  The AutoMacs is user operated.

•   MoFlo - three laser, nine color, high speed sorter with lasers at 488nm, 633nm, and 360nm
•   Aria - a five laser, eighteen color, high speed sorter with lasers at 488nm, 532nm, 405nm, 635nm
•   AutoMacs – Automated benchtop magnetic cell sorter.

Training is mandatory for access to the benchtop analyzers.  Training is $75 and should be scheduled when an experiment is available.


Computers and multiple software packages are available for data analysis free of charge to users.

J. David Farrar, Ph.D.

Location 1:
Jake L. And Nancy B. Hamon Biomedical Research Building
North Campus NA7.300
6000 Harry Hines Blvd.
Dallas, TX 75390-9093
214-648-7325

Location 2:
Cecil H. and Ida Green Biomedical Research building,
South Campus Y7.310
5323 Harry Hines Blvd.
Dallas, TX 75390-9093
214-648-6887

To  Schedule an initial appointment, contact the facility you wish to use.  Once you are trained you may access the schedule at the following link:

http://utswflow.calendarhost.com