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| Test Description |
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Test Name
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FISH, telomere panel
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Synonym(s)
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Telomere FISH
FISH, subtelomere panel
Subtelomere FISH
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Description
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Fluorescence In Situ Hybridization (FISH) is performed to detect microdeletions of the terminal portions of the long and short arms of each chromosome (excluding the short arms of the acrocentric chromosomes 13, 14, 15, 21, and 22).
Note: Cytogenetics, Blood, Constitutional Karyotype must be ordered on the same specimen, unless cytogenetic analysis has been performed on a prior specimen.
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Methodology
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Fluorescently labeled DNA probes specific for the subtelomeric sequences of each chromosome arm (excluding the short arms of chromosomes 13, 14, 15, 21, and 22, for which no specific subtelomeric probes are available) are hybridized to unstained metaphase chromosomes. Cells are scored for the presence or absence of each fluorescent signal.
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Performed
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Daily |
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Turnaround Time
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8-10 Days |
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Specimen Requirements
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Minimum 3 mL aseptically collected blood in a 5 mL green top, sodium heparin tube (or 2 mL pediatric green top, sodium heparin tube). DO NOT use lithium heparin tubes.
Note: Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
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Rejection Criteria
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Frozen, clotted, hemolyzed or grossly contaminated specimens. Incorrect anticoagulant used. |
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CPT Code(s)
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88271x41, 88273x15, 88291 (if routine cytogenetics not requested, add 88230) |
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Reference Range
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See interpretive report |
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Contact
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Cytogenetics Lab: (214) 645-7000
Customer Service: (214) 645-7057 or Toll Free (877) 887-8136
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Last Modified: Feb 12, 2008 |
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