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| Test Description |
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Test Name
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Loss of Heterozygosity (LOH): 1p/19q fragment analysis |
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Synonym(s)
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Oligodendroglioma PCR |
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Description
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Loss of heterozygosity (LOH) involving 1p and 19q is a common genetic alteration in oligodendroglioma, a type of brain tumor. Positive 1p/19q LOH has been associated with chemosensitivity, extended response to chemotherapy, and extended survival. |
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Methodology
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This assay is performed as described (Hatanpaa et al., Lab Invest 83, 419-428, 2003) with small modifications. An area of tumor is identified on an H&E section by a neuropathologist. DNA is extracted from the corresponding area in an unstained paraffin section. Highly polymorphic dinucleotide repeat loci on 1p and 19q are amplified by PCR. Fragment analysis is performed by capillary electrophoresis. |
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Performed
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As requested |
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Turnaround Time
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2-5 days |
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Specimen Requirements
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Paraffin tissue slides, H&E slides, copy of the pathology report. If a neuropathology consultation is requested as well, also send all microscopic slides, brief clinical history, and preoperative radiology report or MRI scans.
Send specimens and other materials to the address below:
Dr. K. Hatanpaa
UT Southwestern Medical Center
Department of Pathology, H2.130
5323 Harry Hines Blvd.
Dallas, TX 75390-9073
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Rejection Criteria
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CPT Code(s)
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83891, 83892x2, 83901x5, 83900, 83907, 83909x5, 83912 |
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Reference Range
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See interpretive report. |
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Contact
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Dr. Kimmo Hatanpaa: (214) 648-2148
Customer Service (214) 645-7057 or Toll Free (877) 887-8136 |
Last Modified: 02/10/2011 |
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