| Test Description |
|
Test Name
|
Paroxysmal Nocturnal Hemoglobinuria Testing (PNH) |
|
Synonym(s)
|
PNH Testing By Flow Cytometry
CD55/CD59/GPI-Linked Antigen testing
|
|
Description
|
GPI-anchored antigens are lacking from a portion of leukocytes, and usually erythrocytes, in patients with Paroxysmal Nocturnal Hemoglobinuria (PNH). This assay uses four-color (six-parameter) flow cytometry to measure the expression of these antigens on various cell populations. |
|
Methodology
|
Flow Cytometry |
|
Performed
|
Monday - Friday. |
|
Turnaround Time
|
Results available next business day. |
|
Specimen Requirements
|
Peripheral Blood:
- One full 10 mL (minimum 5 mL) yellow top tube (ACD Solution A or B) or
- One full 5 mL lavender top (K2-K3EDTA) tube
Transport Instructions:
- Room temperature (20-25°C)
Specimen Stability
- K2-K3EDTA specimens should be processed within 30 hours of collection.
- ACD specimens should be processed within 72 hours of collection.
All tubes must be properly drawn; partial draw may cause cell lysis and loss due to hypertonic osmotic changes.
|
| CPT Codes |
88184, 88185 (x number of markers), 88188 |
| Reference Range |
See interpretive report. |
|
Contact
|
Clinical and Technical Support
Flow Cytometry and Cellular Immunology Laboratory
214-645-7080 or 1-877-887-8136 Ext.7080
Zenaida Rivas, MT (ASCP), Laboratory Manager
Nitin Karandikar, M.D., Ph.D., Medical Director
Customer Service (214) 645-7057 or Toll Free (877) 887-8136
|
