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| Test Description |
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Test Name
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Paroxysmal Nocturnal Hemoglobinuria Testing (PNH) |
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Synonym(s)
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PNH Testing By Flow Cytometry
CD55/CD59/GPI-Linked Antigen testing
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Description
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GPI-anchored antigens are lacking from a portion of leukocytes, and usually erythrocytes, in patients with Paroxysmal Nocturnal Hemoglobinuria (PNH). This assay uses four-color (six-parameter) flow cytometry to measure the expression of 5 of these antigens (i.e., CD14, CD16, CD24, CD55, and CD59) on RBCs, monocytes, and B-lymphocytes. |
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Methodology
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Flow Cytometry |
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Performed
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Monday - Friday. |
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Turnaround Time
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Results available next business day. |
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Specimen Requirements
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Peripheral Blood:
- One full 10 mL (minimum 5 mL) yellow top tube (ACD Solution A or B) is preferred,
- One full 5 mL lavender top (K2-K3EDTA) tube is acceptable, or
- One full 5mL green top (Na Heparin) tube is also acceptable
Transport Instructions:
- Room temperature (20-25°C)
Specimen Stability
- K2-K3EDTA specimens should be processed within 30 hours of collection.
- ACD specimens should be processed within 72 hours of collection.
All tubes must be properly drawn; partial draw may cause cell lysis and loss due to hypertonic osmotic changes.
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| CPT Codes |
88184, 88185 (x number of markers), 88188 |
| Reference Range |
See interpretive report. |
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Contact
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Clinical and Technical Support
Flow Cytometry and Cellular Immunology Laboratory
214-645-7080 or 1-877-887-8136 Ext.7080
Karén Peart, MT(ASCP)SH, Chief Medical Technologist
Nitin Karandikar, M.D., Ph.D., Co-Director
Franklin Fuda, D.O., Co-Director
Customer Service (214) 645-7057 or Toll Free (877) 887-8136
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Last Modified: 02/22/2011
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