Flow Cytometry: T-cell, B-cell or NK cell Quantification

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Test Description
Test Name	T-cell, B-cell or NK cell Quantification
Synonym(s)	Immunodeficiency Evaluation and Monitoring Quantitative Lymphocyte Analysis - Lymphocyte Subsets Quantitative Lymphocyte Analysis - T-Cell Subsets Lymphocyte Subsets T-cell subsets (helper/suppressor ratio) CD4 count for AIDS monitoring CD4 count for HIV monitoring CD4 count CD3 count CD3 count for Transplant monitoring T, B and NK subset quantification
Description	These are a set of quantitative tests used to determine the absolute numbers of various cell types in the blood (e.g., total T cells, CD4 T cells, CD8 T cells, B cells, NK cells). The actual test is determined by the clinical situation. For example, in HIV disease, only a CD4 count is typically monitored. In a workup for immunodeficiency, the physician may request quantification of all subsets. The client provides the total leukocyte (WBC) count and the lymphocyte percentage ("CBC data"). The lab will determine the relative proportion of the requested cell subsets within the lymphocyte population. The absolute count is determined by a simple calculation, based on the CBC data.
Methodology	Flow Cytometry, Dual Platform (CD3/CD4/CD8/CD45) and/or (CD3/CD16 CD56/CD45/CD19)
Performed	Monday - Friday.
Turnaround Time	Results available next business day. Same day results are available if sample is received in the lab by 1:00 pm. Stat service for this testing is available with direct coordination with the laboratory staff (additional charges may apply).
Specimen Requirements	Special Instructions: Please submit WBC count and Percent (%) Lymphs from CBC report from SAME date of collection. SPECIMEN CANNOT BE FROZEN Peripheral Blood: One full 5 mL (2.5 mL minimum) lavender top tube (K₂-K₃EDTA) is preferred, or One full 3 mL ACD is an acceptable alternative anticoagulant, but not when used in Plasma Separator Tube (PST). Transport Instructions: Room temperature (20-25°C) Specimen Stability K₂-K₃EDTA specimens should be processed within 30 hours of collection. ACD specimens should be processed within 72 hours of collection. All tubes must be properly drawn; partial draw may cause cell lysis and loss due to hypertonic osmotic changes.
CPT Codes	CD3 quantification: 86359 CD4 quantification: 86361 T cell subset: 86359, 86361 T cell and B cell subset: 86359, 86360, 86355, 86357 B cell subset: 86355 and 86357 NK cell subset: 86357
Reference Range	Ranges based on a study that evaluated a large number of healthy donors (Shearer, et al, J. Allergy Clin Immunol, 2003, 112(5):972-80).
Contact	Clinical and Technical Support Flow Cytometry and Cellular Immunology Laboratory 214-645-7080 or 1-877-887-8136 Ext.7080 Zenaida Rivas, MT (ASCP), Laboratory Manager Nitin Karandikar, M.D., Ph.D., Medical Director Customer Service (214) 645-7057 or Toll Free (877) 887-8136

Last Modified: May 8, 2008