| Test Description |
|
Test Name
|
Lymphoblastoid Cell Line, Establishment Of |
|
Synonym(s)
|
|
|
Description
|
Immortalization of white blood cell line. |
|
Methodology
|
Lymphocytes are separated from peripheral blood and cultured in medium with Epstein-Barr virus until B-cell transformation occurs. |
|
Performed
|
Daily |
|
Turnaround Time
|
2 - 4 weeks; dependent on growth rate of cultured cells. |
|
Specimen Requirements
|
20 mL of aseptically collected blood in green top sodium heparin tubes; from a child, a minimum of 5 mL of blood may be submitted.
Note: Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
|
| Rejection Criteria |
Frozen, clotted, hemolyzed or grossly contaminated specimens. Incorrect anticoagulant used. |
|
CPT Code(s)
|
99199 |
|
Reference Range
|
See interpretive report. |
|
Contact
|
Cytogenetics Lab: (214) 645-7000
Customer Service: (214) 645-7057 or Toll Free (877) 887-8136 |