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| Test Description |
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Test Name
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Chromosome Analysis, Solid Tissue, Non-Neoplastic |
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Synonym(s)
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Cytogenetics, Solid Tissue, Non-Neoplastic
Karyotype, Solid Tissue, Non-Neoplastic
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Description
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Cytogenetic analysis of cultured fibroblasts to assess for the presence of constitutional (congenital) chromosome aberrations. |
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Methodology
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Tissue is minced and/or enzymatically disaggregated and is cultured until sufficient dividing cells are present (10 days to 4 weeks). Standard cytogenetic harvest. G-banding with trypsin-Wright stain. The chromosomes in twenty metaphase spreads are counted, five cells are fully analyzed and two are karyotyped. |
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Performed
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Daily |
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Turnaround Time
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2 - 3 weeks (dependent on growth rate of cultured cells). |
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Specimen Requirements
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At least 5mm3 of aseptically collected fresh tissue from surgery or autopsy, or a generous 2 mm diameter (minimum) full thickness skin biopsy. Do not send skin from autopsy. Kidney, lung, fascia, cartilage and chorionic villi are preferred specimens from autopsy. Place specimen in 10 ml of sterile tissue culture medium and refrigerate until transported. Do not freeze. Do not put in formalin or other fixative.
Note: Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
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Rejection Criteria
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Frozen, fixed or grossly contaminated specimens. Specimens sent without tissue culture medium. |
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CPT Code(s)
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88233, 88262, 88291 |
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Reference Range
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See interpretive report. |
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Contact
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Cytogenetics Lab: (214) 645-7000
Customer Service: (214) 645-7057 or Toll Free (877) 887-8136 |
Last Modified: May 2, 2007 |
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