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| Test Description |
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Test Name
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Chromosome Analysis, Percutaneous Umbilical Cord Blood Sample (PUBS) (STAT) |
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Synonym(s)
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Cytogenetics, Percutaneous Umbilical Cord Blood Sample (PUBS) (STAT)
Karyotype, Percutaneous Umbilical Cord Blood Sample (PUBS) (STAT)
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Description
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Cytogenetic analysis of fetal blood obtained by cordocentesis to assess for the presence of constitutional (congenital) chromosome aberrations. |
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Methodology
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48- and 72-hour cultures with phytohemagglutinin, standard cytogenetic harvest, G-banding with trypsin-Wright stain. The chromosomes in 20 metaphase or prometaphase cells are counted, 5 cells are fully analyzed, and 2 are karyotyped. |
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Performed
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Daily. |
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Turnaround Time
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48 - 72 hours. |
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Specimen Requirements
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1-2 mL aseptically collected blood in a pediatric green top, sodium heparin tube. DO NOT use lithium heparin tubes. Must be tested to verify fetal origin.
Note: Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
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Rejection Criteria
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Frozen, clotted, hemolyzed or grossly contaminated specimens. Incorrect anticoagulant used. |
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CPT Code(s)
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88230-22, 88262, 88291 |
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Reference Range
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See interpretive report. |
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Contact
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Cytogenetics Lab: (214) 645-7000
Customer Service: (214) 645-7057 or Toll Free (877) 887-8136 |
Last Modified: May 2, 2007 |
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