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| Test Description |
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Test Name
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Chromosome Analysis, Solid Tumor |
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Synonym(s)
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Cytogenetics, Solid Tumor
Karyotype, Solid Tumor
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Description
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Cytogenetic analysis of cultured cells obtained from tumor or suspected tumor to assess for the presence of neoplastic clones. |
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Methodology
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Specimen is minced and/or enzymatically disaggregated, followed by a direct harvest and/or harvest after overnight or longer culture, followed by G-banding with trypsin-Wright stain. Twenty metaphase spreads are fully analyzed and two karyotypes are prepared. An additional two karyotypes are prepared for each abnormal clone observed. |
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Performed
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Daily. |
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Turnaround Time
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10 days - 3 weeks. |
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Specimen Requirements
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At least 5mm3 of aseptically collected, fresh, non-necrotic tumor or lymph node. Put specimen in 10 ml of sterile tissue culture medium immediately. Do not put in formalin or other fixative. Oncologic diagnosis (suspected or confirmed) must be provided.
Note: Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
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Rejection Criteria
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Frozen, fixed or grossly contaminated specimens. Specimens sent without tissue culture medium. |
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CPT Code(s)
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88239, 88262, 88291
More than 20 cells counted: add 88285
More than 2 karyotypes: add 88280
Fewer than 15 cells counted: 88239, 88261, 88280, 88285, 88291
No cells available for analysis, or study cancelled after initiation of culture: 88239 |
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Reference Range
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See interpretive report. |
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Contact
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Cytogenetics Lab: (214) 645-7000
Customer Service: (214) 645-7057 or Toll Free (877) 887-8136 |
Last Modified: May 2, 2007 |
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