| Test Description |
|
Test Name
|
Chromosome Analysis, Amniotic Fluid |
|
Synonym(s)
|
Cytogenetics, Amniotic Fluid
Karyotype, Amniotic Fluid
|
|
Description
|
Cytogenetic analysis of amniocytes for prenatal diagnosis of chromosome abnormalities. |
|
Methodology
|
Amniotic fluid is cultured in four to six vessels, then harvested in situ and G-banded with trypsin-Wright stain. The chromosomes in fifteen metaphase spreads from fifteen colonies in at least 2 separate cultures are counted. Five cells are fully analyzed, and two are karyotyped. |
|
Performed
|
Daily. |
|
Turnaround Time
|
7 - 9 days. |
|
Specimen Requirements
|
Aseptically handled amniotic fluid in a tightly capped, sterile tissue culture tube. Seal tightly with Parafilm. For pregnancies 16 weeks or more, 20 ml of fluid should be submitted. Less may be submitted for pregnancies of 12 - 15 weeks.
Note: Deliver to lab at room temperature within 24 hours. If collected over a weekend, call the laboratory to make arrangements for specimen delivery. May be refrigerated if not transported immediately. Do not freeze. Protect from heat or cold.
|
| Rejection Criteria |
Frozen or grossly contaminated specimens. |
|
CPT Code(s)
|
88235, 88269, 88280, 88291 |
|
Reference Range
|
See interpretive report |
|
Contact
|
Cytogenetics Lab: (214) 645-7000
Customer Service: (214) 645-7057 or Toll Free (877) 887-8136 |
