Fees for Service
The UTSW TTC will cryopreserve gametes, fertilized eggs, and ovaries from transgenic and knock-out strains of mice. The Center will store the cryopreserved gametes. The Center will, on request, thaw the cryopreserved bisected ovaries and surgically transfer the ovary to histocompatible recipients. The Center will, on request, recover the cryopreserved embryos and transfer them to pseudopregnant recipients.
Cryopreservation – procedure $600 – embryos
$300 – ovaries
Cryopreservation – storage $100/year
Recover and transfer embryos $600 – 4 transfers
Recover and transfer ovaries $1,000 – 4 transfers
Guarantee Policy
In exchange for the fees charged for services, the Center warrants that it will cryopreserve 200 embryos or 6 ovaries from transgenic or knock-out mice. The embryos will be stored in cryovials at ~35/vial. 10 to 14 embryos will be stored in a vial for quality assurance. The bissected ovaries will be stored 1/2 ovary/vial.
Investigator Requirements
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Investigator must submit the Animal Reassignment Request Form for females carrying transferred eggs or ovaries to be transferred and housed in the ARC quarantine.
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The Investigator contacts the Center manager, John Ritter (x56287) to discuss and schedule the cryopreservation.
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The Investigator will transfer to the TTC, 6 to 8 proven stud males between 8 to 14 weeks of age. The Center will not accept unproven males or senescent males for this service. The males birthdate, genetic background and genotype must be provided. Following the cryopreservation, the males can be returned to the Investigator’s colony but they will not be considered SPF animals.
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The TTC staff will perform the hormone priming of WT females (B6, 129SvEv (S6), B6SJLF1), and set up appropriate matings. If the Investigator prefers to cryopreserve the line in a homozygous condition, the Investigator will need to supply the females of the appropriate number and age to perform the procedure. Please consult with the TTC staff to arrange this alternative.
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