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DNA Sequencing Variation/SNP Genotyping Facility
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| Description: |
This is a core facility for allelic discrimination using an ABI 7900HT real time PCR instrument and the TaqMan Assay. |
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Director:
Contact:
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Bill Crider, Ph.D.
Billy.Crider@utsouthwestern.edu
214-648-5101
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| Facility Location: |
McDermott Center for Human Growth & Development
Simmons Biomedical Research Building
North Campus/NA2.138
6000 Harry Hines Blvd.
Dallas, TX 75390-8591 |
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| Pricing: |
The cost of each SNP assays is $0.30 plus the cost of the ABI probes. The cost of the ABI probes should be around $0.50 per sample. There are several options regarding the ordering of the TaqMan probes from ABI. Probes can be ordered from ABI’s extensive list of pre-designed probes (Assays-By-Design) or ABI can design and test probes (Assays-On-Demand) if your SNP is not included in this list. A third option is to design and order the probes yourself. Each method has associated benefits and costs. See the ABI home page for more information: http://home.appliedbiosystems.com |
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| Sample Requirements: |
We need 3 ul of genomic DNA (10 ng/ul) for each SNP you wish to have assayed |
